Gene detail

Gene detail information of Glyur001390s00030763.2

Functional Annotation

Database	Orthologs	E value	Annotation
TAIR	AT1G80560.1	7.00E-167	isopropylmalate dehydrogenase 2
NR	XP_004486631.1	0	PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic-like [Cicer arietinum]
COG	YP_007079545.1	2.00E-159	3-isopropylmalate dehydrogenase [Pleurocapsa sp. PCC 7327]
Swissprot	tr\|P29102\|LEU3_BRANA	0	3-isopropylmalate dehydrogenase, chloroplastic (Precursor)
trEMBL	tr\|G7IMC1\|G7IMC1_MEDTR	0	3-isopropylmalate dehydrogenase {ECO:0000256\|RuleBase:RU004445}

Sequence

Transcript Sequence

>Glyur001390s00030763.2
GTACTAAAAAGAACACAAAAGAAAGATAACTACTGTACATCACTCGTAGACGGACAGTGTGAGTGAGTGTGGTCTGCGGCGGAGTTCTTGTAGTTGACTCGTTGCAGCAACCAAAAGAGAGAGAAAAAAATGGCGGCTGGTCTGCAAGTGCAACTCCTCCACGCGAGACCCTTTCAGTCTCGTTCNCTATCGTTGCCGGCGAGACTAACTTCCTTTAGGTGCACGTGTTCCGCTTCTACCCCTTCTTCCAAACAACGCTCCTACAATATCACTCTCCTTCCCGGCGACGGTATCGGCCCNGAAGTCATCTCCGTCGCTAAAGACGTTCTTCTCCTCACCGGATCTCTCCACGGGATCAAACTTGAGTTCCAAGAGATGCCTTTGGGTGGAGCCGCGCTTGATGCTACTNGAGTCCCCTATAAATGGGATAAAAACGAGAAACATCTGAAGCCNGAGACTGGCTTGCTTCAGCTACGAGAAGGGCTTCAAGTATTTGCAAATCTGAGACCAGCAACGGTGTTCCCACAGTTAGTGGATGCTTCAACTTTGAAGAGAGAGGTGGCTGAGGGGGTTGATCTCATGGTTGTAAGGGAACTAACCGGAGGTATCTATTTTGGTAAACCTAGGGGATTTGGTACCAATGAACATGGTGAAGAGACTGGCTTTAATACTGAGATTTATGCTGCACATGAGATTGATCGCATAGCTCGTGTTGCATTTAACATTGCTCGGAAGCGTCANGGGAAACTTTGCTCTGTTGATAAAGCCAATGTACTAGAGGCATCTATGTTCTGGAGGAAAAGAATTTTGGCATTAGCACAAGAATATTCTGACNTTGAGCTCTCACACATGTATGTTGATAATGCCGCGATGCAACTAGTTCGNTATCCAAAACAGTTTGACACTATTGTAACAAACAACATTTTTGGCGATATATTATCCGATGAGGCTTCAATGATCACCGGAAGTATTGGGATGCTTCCTTCCGCTAGCCTAGGGGATTCGGGACCTGGACTTTTTGAACCTATTCATGGTTCTGCACCTGATATTGCTGGACAGGACAAGGCAAACCCATTTGCTACTGTTCTTAGTGCTGCNATGCTTTTGAAATATGGTCTTGGAGANGANGAGGCCGCNGAAAGAATAGAGAATGCAGTTCTGGACACTTTGAACCGGGGATTTCGGACTGCTGACATATATTCTGCNGGAACAAATCTGGTAGGATGCAAACAACTAGGTGAAGAGATACTGAAGTCAGTTGAATCTCACGCTCCTGCTGCTGCTGCTGCTGCAGTGTGATTTAATACCAACCAACCGAACGCGACATAGAATTTCAGGGGCTGGTTTCTCTTGGATACCACCATGACTATCAAAGCTGAAAAAAAGTATCCCTAGTCTTTGAGAGCCTTTGCTTCCATCTGCATTGTGTATGTTGGTTTCAACACCAAGGTTCATATGCGAAGCTGCTTGTAATAATAGTGGAATAATGAATGATATAGATGTTGGAATTTTCATTGCCATTCTAAAAACTGAGAAACTACTTTTAACATAGGACATCAATATGGGGCATGCACACAACACAGACACGGATATTCCAAAATTTAAGATGTCTCGTATGATTTGAAGCGGT

Protein Sequence

Location information (JBrowse)

Scaffold	Start	End	Strand
Scaffold01390	16083	21050	+

Gene family

Gene family	subfamily
--	--

KEGG pathway

KO	Enzyme	Enzyme ID	pathway	mapID
K00052	leuB	EC:1.1.1.85	2-Oxocarboxylic acid metabolism	map01210
			2-Oxocarboxylic acid metabolism	map01210
			Biosynthesis of amino acids	map01230
			Biosynthesis of amino acids	map01230
			C5-Branched dibasic acid metabolism	map00660
			C5-Branched dibasic acid metabolism	map00660
			Valine, leucine and isoleucine biosynthesis	map00290
			Valine, leucine and isoleucine biosynthesis	map00290

Gene Ontology

biological_process

GO:0009098 leucine biosynthetic process

GO:0055114 oxidation-reduction process

cellular_component
GO:0005737 cytoplasm

molecular_function

GO:0000287 magnesium ion binding

GO:0003862 3-isopropylmalate dehydrogenase activity

GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

GO:0051287 NAD binding

Pfam domain

Protein	Pfam accession	Pfam name	Alignment start	Alignment end	E-value
Glyur001390s00030763.2	PF00180.15	Iso_dh	46	94	2.00E-07
Glyur001390s00030763.2	PF00180.15	Iso_dh	103	373	9.00E-109

Expression pattern

SRP053019

SRA Experiment	Description	TPM
SRR1783599	control	71.053
SRR1783600	control	70.992
SRR1783602	moderate drought stress	72.822
SRR1811619	moderate drought stress	71.673

DRP000996

SRA Experiment	Description	TPM
DRR006519	Root Summer glycyrrhizin high producing strain	54.450
DRR006520	Root Summer glycyrrhizin high producing strain	53.542
DRR006521	Root Winter glycyrrhizin high producing strain	12.046
DRR006522	Root Winter glycyrrhizin high producing strain	11.723
DRR006523	Root Summer glycyrrhizin low producing strain	61.409
DRR006524	Root Summer glycyrrhizin low producing strain	60.654
DRR006525	Leaf Summer glycyrrhizin high producing strain	37.468
DRR006526	Leaf Summer glycyrrhizin high producing strain	36.440

SRP065514

SRA Experiment	Description	TPM
SRR2867873	Salt Stress (root)	84.308
SRR2868004	Drought Stress (root)	58.319
SRR2967015	Control (root)	64.714

SRP215420

SRA Experiment	Description	TPM
SRR9715738	Leaf	35.971
SRR9715740	Leaf	37.215
SRR9715741	Leaf	38.979
SRR9715739	Root	36.514
SRR9715742	Root	44.524
SRR9715743	Root	43.787

SRP188776

SRA Experiment	Description	TPM
SRR8749027	MeJA induced 9h	160.277
SRR8749028	MeJA induced 9h	73.646
SRR8749029	MeJA induced 9h	107.409
SRR8749030	Uninduced	32.607
SRR8749031	Uninduced	18.462
SRR8749032	Uninduced	43.447

SRP175571

SRA Experiment	Description	TPM
SRR8400026	NaCl treatment - Root	59.264
SRR8400027	Control - Root	57.508