Gene detail

Gene detail information of Glyur000158s00011356

Functional Annotation

Database	Orthologs	E value	Annotation
TAIR	AT1G03310.2	0	debranching enzyme 1
NR	XP_004493650.1	0	PREDICTED: isoamylase 2, chloroplastic-like [Cicer arietinum]
COG	YP_003690956.1	2.00E-106	glycogen debranching enzyme GlgX [Desulfurivibrio alkaliphilus AHT2]
Swissprot	tr\|Q8L735\|ISOA2_ARATH	0	Isoamylase 2, chloroplastic (Precursor)
trEMBL	tr\|A0A072U1Z3\|A0A072U1Z3_MEDTR	0	Isoamylase-type starch debranching enzyme {ECO:0000313\|EMBL:KEH23729.1}

Sequence

Transcript Sequence

>Glyur000158s00011356.1
ATGGCAAATCTCGTGCCATCACTCTCATTCTCAGTGATTCCCTGGAATCAGCAGCATAAACACGGAACCAAATGTTCCTTCTTTCACAACTATTCCTTTACCCAAAGGAAACAGCAGCATCAACTTGTTAGTAGGAATTTGCAGGATCTCACCAACCCCTTTTCCCAGAATCTCACTACAAAATTGTGCGCCACGTCACGGTTGTCCGTCGAAGAAACCGAGCAAAAAATCACTCCCTTTACCCAATCCGAAGACCTAAAAACGGCATTGCCGTATTTGTTTCGGACAGAAACCGGTGACGGTTTGGTCAGAGTTTATGTTACAAAGAAAAAAGTTAGTTATTCTGTCTATATTGAGATTTCTTCGTTGGAGTTAAGTGGTGGTGAAGGGGAAGCACTGGTGTTGTGTTGGGGTGTGTATAGAGTCGATTCATCGAGTGTAGAAGCGATGAATGTGACCCCATTGNTGCAAACTTCTCAGGGAAAGTTTGCAGTTGAATTGGAATTTGATGCTAGACAGGTTCCGTTGTACTTGTCGTTTTTGTTGAGATCATCGTTGGAAATCAGAAGCCATAGGAAGAGGAATTTCTGTGTGCCTGTTGGTTCTCTTCCGGGTTCCCCATCTCCACTGGGGCTCTCTTTTTCCCCTGACGGGTCTATGAATTTTGCGGTTTTCTCGAGACACGCAGAGGGTGTGGTTTTGTGCTTGTATTATGATGATGATGATGATGATGATGATGATGATGACACTAGTGTGGAGGAGAAGAAGCCTGCTTTGGAGCTTGACCTGGATCCTTATGTAAATAAGTCAGGGGATATCTGGCATATCTCCTTTGAGAGTGCCTTGAATTTTGTGAGTTATGGCTATCGCTTTCTTCACACAAACAAGGATGATAAGGGTGTTGTTTTGGATCCCTATGCTAGGATTGTTGGGAATTCTCTTCCTAATGGTGTTGGGTCAGTGAAGAAGAACCTTGGATGTTTGGGAAAGGAACCTGCTTTTGACTGGGGTGATGATTATCATCCCAATTTGGCCATGGAGGAACTAGTGGTGTATCGGTTGAATGTGAAGCGCTTTACGGAGCACGAGTCCAGCCAACTTTCCAGTGATTTAGCTGGGACCTTTTCTGGTTTGGCTAAAAAGCTGCAGCATTTTAAAGATCTGGGTGTGAATGCTGTTCTGTTGGAGCCGGTTTTCACATTTGATGAGGAAAAGGGGGCATATTTTCCTTGTCATTTTTTCTCTCCAATGAACCTTTATGGGCCATCTGGTGGTCCTGTATCTACTNTAACCTCTATGAAAGAGATGGTGAAGACTATGCATGCCAATGGGATAGAAGTTCTCATCGAAGTTGTTTTCTCCAATACCGCTGACACTGGTGCTCTGCAAGGTATTGATGACTTGTCTTACTACTATGCCAATGGAGTTGGTGATTTGAAGGTACACAGTGCTTTGAATTGTAACTATCCTATAGTGCAAAATTTGATTCTGGATAGCCTTCGGCATTGGGTGACCGAGTTTCACATTGATGGCTTCTCTTTTGTGAATGCTTCACATCTGTTGAGGGGTTTTCATGGAGAACACTTGTCTCGACCTCCCTCGGTTGAAGCAATTGCCTTCGACCCAGTACTCTGGAAGACTAAAATCATTGCAGATTGCTGGGATCCCCATGACATGGTAGCAAAGGAGACTCGCTTTCCTCATTGGATGAGATGGGCTGAAATAAACACAAACTTTTGCAATGATGTGAGAAACTTTTTAAGGGGTGAAAATCTTCTTAGCAACCTTGCAACAAGACTTTGTGGTAGTGGGGATATGTTTTCTGATGGAAGGGGCCCTGCATTCTCTTTGAACTACATTGCCAGGAATTTTGGACTCTCTCTCGTGGACATAGTCAGCTTCAGCAGTGATGATGTAGCTGCAGAATTGAGTTGGAATTGCGGAGAAGAAGGGCCTACGAATAACAGAACTGTTCTTGAAAGACGACTCAAACAAATCCGCAATTTCCTCTTCATCTTGTTCATGTCATTAGGAGTACCTGTTCTGAACATGGGAGATGAGTGTGGTCAGTCTTCTGATGGTTCTCCTGCATATGGTGACATGAAACCCTTCAANTGGGTTACCTTAAAAACAGGTTTTGGTAAACAAACCACACAATTCATTTCCTTTTTGACTTCATTGAGAAGGCGGCGGAGTGATCTTCTTCAGAGTAGGAGCTTCTTAAAAGAGGAAAACATCGAATGGCACGGAGCTGACCAGGCTCCACCAAGATGGGAAGACCCATCCTGCAAGTTCCTGGCCATGACTTTGAAGGCTGAAGAAAGGGAGTTCCAAGAGAGCAGCTCTGTATCTTCTGATATATTAGGGGATTTATTTATTGCTTTCAATGCAGCTGATCATCCAGAAACTGTAGTTTTACCCTTCCCTCCAGAAGGGATGTCGTGGTACCGCCTTGTTGATACAGCTCTTCCATTTCCTGGGTTTTTCTTGACCAATGGTGAATTAGTACCCGAGGAAATACCAGGTTTGTCTACTTATCAAATGAAGCCTTACAGCTGCACTTTATTTGAGGCAAAGAATCATACCAGATAG

Protein Sequence

Location information (JBrowse)

Scaffold	Start	End	Strand
Scaffold00158	132376	134967	-

Gene family

Gene family	subfamily
Carbohydrate-Active enzymes Family	CBM48,GH13

KEGG pathway

KO	Enzyme	Enzyme ID	pathway	mapID
K01214	ISA, treX	EC:3.2.1.68	Starch and sucrose metabolism	map00500

Gene Ontology

biological_process
GO:0005975 carbohydrate metabolic process

molecular_function

GO:0003824 catalytic activity

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

GO:0043169 cation binding

Pfam domain

Protein	Pfam accession	Pfam name	Alignment start	Alignment end	E-value
Glyur000158s00011356.1	PF02922.13	CBM_48	209	307	7.60E-10
Glyur000158s00011356.1	PF00128.19	Alpha-amylase	373	459	1.50E-07

Expression pattern

SRP053019

SRA Experiment	Description	TPM
SRR1783599	control	31.335
SRR1783600	control	31.132
SRR1783602	moderate drought stress	30.387
SRR1811619	moderate drought stress	30.693

DRP000996

SRA Experiment	Description	TPM
DRR006519	Root Summer glycyrrhizin high producing strain	27.000
DRR006520	Root Summer glycyrrhizin high producing strain	26.581
DRR006521	Root Winter glycyrrhizin high producing strain	1.677
DRR006522	Root Winter glycyrrhizin high producing strain	1.598
DRR006523	Root Summer glycyrrhizin low producing strain	12.866
DRR006524	Root Summer glycyrrhizin low producing strain	12.677
DRR006525	Leaf Summer glycyrrhizin high producing strain	4.621
DRR006526	Leaf Summer glycyrrhizin high producing strain	4.730

SRP065514

SRA Experiment	Description	TPM
SRR2867873	Salt Stress (root)	9.901
SRR2868004	Drought Stress (root)	7.017
SRR2967015	Control (root)	4.879

SRP215420

SRA Experiment	Description	TPM
SRR9715738	Leaf	2.162
SRR9715740	Leaf	2.138
SRR9715741	Leaf	1.077
SRR9715739	Root	39.474
SRR9715742	Root	24.128
SRR9715743	Root	25.643

SRP188776

SRA Experiment	Description	TPM
SRR8749027	MeJA induced 9h	0.581
SRR8749028	MeJA induced 9h	0.658
SRR8749029	MeJA induced 9h	0.386
SRR8749030	Uninduced	0.499
SRR8749031	Uninduced	0.015
SRR8749032	Uninduced	0.120

SRP175571

SRA Experiment	Description	TPM
SRR8400026	NaCl treatment - Root	25.989
SRR8400027	Control - Root	26.903