Gene detail

Gene detail information of Glyur000152s00012715

Functional Annotation

Database	Orthologs	E value	Annotation
TAIR	AT5G49030.3	0	tRNA synthetase class I (I, L, M and V) family protein
NR	XP_004487851.1	0	PREDICTED: isoleucine--tRNA ligase-like [Cicer arietinum]
COG	YP_005071287.1	0	isoleucyl-tRNA synthetase [Arthrospira platensis NIES-39]
Swissprot	tr\|P93736\|SYV_ARATH	1.00E-31	Valine--tRNA ligase
trEMBL	tr\|A0A072V611\|A0A072V611_MEDTR	0	Isoleucine-tRNA ligase {ECO:0000313\|EMBL:KEH37262.1}

Sequence

Transcript Sequence

>Glyur000152s00012715.1
CGGTTGTGCATTTGCAAGTTCAATGGGTAGTGTTAGTGCAGCAACCAAACGAGTGGAGTAGAGTCCTTCTCTTCTCTCAAACTCAAACCAAACCCAATGGAAACAACCATCGCCCTCTTCATGCAAACCTCTTCTTACAGGGTGCTCTCGCGAAATGCTTGTTCATCCTCCAGGAGAACTAATTCCATTGGCTTGTTTTATTCCCGGGGAATCTCATCAGCTAAAGTAGTATCTCTCTCAAACAGGCCAAACTACTGCACTCATTCAAGGGACGATATCAGTTCNTCAAAGCGAAGATCACGGGGGCCTGTTATGGCAGGAAAAAAGGCTGCGGAAGGAATCAAGCAAGAAGATGGTAAATACAAGCACACAGTTGATCTTCCTAAGACAACATTTGGTATGAGGGCAAATTCTTCAGTAAGGGAGCCAGAAATTCAGAAAATATGGGAGGAGAATCAAGTATTTAAAAGAGTTGTTGACAAAAATAGCGGAGGAAATTTCATTCTTCATGATGGTCCTCCATATGCTAATGGTGATCTTCACATTGGCCATGCCTTGAATAAAATTTTGAAGGATATTATAAATCGTTATAAGTCACTGGATCAGGAGGCTAGAAATAATCTTACACCATTGAAATTGAGAGCAAAGGCTGCTAAATTTGCCAAAGAAACTGTTAAAACTCAGATGTCATCTTTTAAGCGCTTTGGAGTATGGGCAGACTGGAATAATCCCTATCTTACTCTAGATCCAAAATATGAAGCTGCCCAGATTGAAGTATTTGGCCAGATGGCCTTGAAAGGCTTTATCTACAGAGGCAGAAAACCTGTCCACTGGAGTCCCTCGTCCCAGACAGCACTTGCTGAGGCTGAGTTGGAGTACCCTGAAGGGCATGTTTCAAGAAGCATATATGCCATTTTCAGAATTGCAAGTGCTCCTCTAGTGCCAAGTGACCTACTACAAGAATTTCCAAATTTGTGCTTGGCCATTTGGACTACAACTCCCTGGACTATTCCTGCCAACGCAGCTGTTGCAGTAAATTCCAAGCTTGAATACGATGTTGTTGAAGTGGAGTCACTAAATGGACATGCCTCTTCATCTGGTGAAACTAGAAAGAAAAGGCTTGGAGTTGTTTTAAAGGATGAAAAAATGCTGTTTCTTATTGTAGCTTCAGAACTTGTGCCAAAGTTAGAGGCAAAATGGGGTGTGAAGCTTGTTGTCAAGAGGAGACTGTTAGGTTCTGACTTGGAAAACTACAGATACATCCATCCAGTAGATAATAGGGAATGTCCTGTCGTAATTGGGGGAGATTATATTACAACAGAAACAGGAACAGGACTGGTCCATACAGCTCCTGGACATGGTCAAGAAGATTATGTGACTGGTCAGAAGTATGGGCTACCCATACTATCTCCGGTAGATGATAAGGGAATATTCACTGAAGAAGCTGGGCAATTTAGTGGGCTCAATGTTCTTGGTGAAGGCAACACTGCTGTTGTCAAATATTTGGATGAAAATCTGTCACTCATCATGGAAGAATCATATGAACACAAGTATCCATATGATTGGCGAACAAAAAAACCAACAATATTTAGAGCAACAGAGCAATGGTTTGCATCGGTTGAGGGATTTCGCCAAGCTGCCATGGATGCTATTGGGCATGTGAAATGGGTTCCACCTCAGGGAGAAAACAGAATCTCAGCAATGACCTCTAACCGNTCTGACTGGTGTATATCACGGCAAAGGACATGGGGTGTCCCTATTCCAGTTTTTTATCATCTGCAGTCTGGAGAACCTCTTATGAATGAAGAGACAATTGATCATATAAAATCTATTATAGCCCAAAAGGGTAGTGATGCATGGTGGTACATGACAGTGGAGGAACTTCTCCCAGCTAAATATCGTGATAAAGCTGCAGAATATGAAAAAGGAACTGACACAATGGATGTATGGTTTGATTCAGGTTCCTCCTGGGCTGCAGTCTTGGGAGAAAGAGATTCCCTTAGCTTTCCTGCAGACTTGTATCTTGAAGGAACAGATCAACATCGAGGTTGGTTTCAGAGTTCATTGCTAACGAGTATAGCTACAACAGGAAAGGCTCCGTATTCTAGTGTTATAACTCATGGATTTGTATTGGATGAGAAGGGCTTAAAGATGAGCAAGTCTTTGGGTAATGTTGTGAATCCACGTAGTGTGATTGAAGGAGGAAAAAATCAGAAGGAAGCACCTGCATATGGAGCTGATGTCCTCCGACTCTGGGTTTCTAGTGTAGATTACTCCGGTGATGTGATGATTGGCCCTCAGATTCTTCGTCAAATATCTGAAATTTACAGGAAGTTACGGGGAACTTTGAGATATCTCTTGGCAAACCTTCATGATTGGAAAACAGATTATACTGTTAAATACAATGAACTTCCCAGAATTGATCAGCATGCGCTATTTCAGCTTGAAAATGTTGTGAAAAGCATTCAAGGGAATTATGAAAATTACCAGTTTTTCAAAATATTTCAGATTTTACAGAGGTTTGTCATTGTTGACCTTTCAAATTTCTATTTTGATGTTGCCAAAGATCGACTTTATGTTGGTGGATCGACGAGTCATACAAGGAGAAGTTGCCAAACAGTTCTTGCAAATCATCTCCTTTCTATTGTGAGAGTAATAGCACCAATATTGCCCCATTTAGCTGAGGATGTGTGGCAGAATCTTCCCTTTCAGTATACAACTGAATATGGTTCCTTTGCTGAATATGTATTCGAATCAAGATGGCCAACCTTGAATGAAAGATGGCTTGCTCTCCCTGTTGAAGAAATTGAATTCTGGGAAAAATTTCTAGAGCTGAGAACAGAGGTGAATAGAGTGTTGGAGGTTGCTCGAACTGGCAAATTAATTGGTGCCAATTTAGATGCCAAGGTTCACATTTATACATCCGACGCCATCATGGCATCCCAACTATCTGAACTATGTGCAACCAAAATCGATGCTGATACATTGCAGCGGTTATTCATAACGTCTCAGGTTGAGATTCTTCCTTCATTGGAAGATGAACATATTGTAAACATACCATATAGTGGAGAATGCCTAATTCAGGGGAAGAGTAAAGTGTGGATTGGCATATCCCGTGCTAGTGGTTCCAAATGTGAAAGATGTTGGAACTATTCACACCAGGTTGGTTCATTTTCAGACCACCCTACCCTCTGCAGCCGCTGCTATGATGTTGTTGCTGTTCATGTGCCCCCTGAAGTAGCTGCAGTCAGTTGAGATTTCAGGACATGCAACTTTTTGTTTAAATTCNAACTTCAATTGTTTTCTGATTAAAAATATTTAGTTTCTTTTTGAAATTGAATTTT

Protein Sequence

>Glyur000152s00012715.1
METTIALFMQTSSYRVLSRNACSSSRRTNSIGLFYSRGISSAKVVSLSNRPNYCTHSRDDISSSKRRSRGPVMAGKKAAEGIKQEDGKYKHTVDLPKTTFGMRANSSVREPEIQKIWEENQVFKRVVDKNSGGNFILHDGPPYANGDLHIGHALNKILKDIINRYKSLDQEARNNLTPLKLRAKAAKFAKETVKTQMSSFKRFGVWADWNNPYLTLDPKYEAAQIEVFGQMALKGFIYRGRKPVHWSPSSQTALAEAELEYPEGHVSRSIYAIFRIASAPLVPSDLLQEFPNLCLAIWTTTPWTIPANAAVAVNSKLEYDVVEVESLNGHASSSGETRKKRLGVVLKDEKMLFLIVASELVPKLEAKWGVKLVVKRRLLGSDLENYRYIHPVDNRECPVVIGGDYITTETGTGLVHTAPGHGQEDYVTGQKYGLPILSPVDDKGIFTEEAGQFSGLNVLGEGNTAVVKYLDENLSLIMEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGHVKWVPPQGENRISAMTSNRSDWCISRQRTWGVPIPVFYHLQSGEPLMNEETIDHIKSIIAQKGSDAWWYMTVEELLPAKYRDKAAEYEKGTDTMDVWFDSGSSWAAVLGERDSLSFPADLYLEGTDQHRGWFQSSLLTSIATTGKAPYSSVITHGFVLDEKGLKMSKSLGNVVNPRSVIEGGKNQKEAPAYGADVLRLWVSSVDYSGDVMIGPQILRQISEIYRKLRGTLRYLLANLHDWKTDYTVKYNELPRIDQHALFQLENVVKSIQGNYENYQFFKIFQILQRFVIVDLSNFYFDVAKDRLYVGGSTSHTRRSCQTVLANHLLSIVRVIAPILPHLAEDVWQNLPFQYTTEYGSFAEYVFESRWPTLNERWLALPVEEIEFWEKFLELRTEVNRVLEVARTGKLIGANLDAKVHIYTSDAIMASQLSELCATKIDADTLQRLFITSQVEILPSLEDEHIVNIPYSGECLIQGKSKVWIGISRASGSKCERCWNYSHQVGSFSDHPTLCSRCYDVVAVHVPPEVAAVS

Location information (JBrowse)

Scaffold	Start	End	Strand
Scaffold00152	164056	173445	-

Gene family

Gene family	subfamily
--	--

KEGG pathway

KO	Enzyme	Enzyme ID	pathway	mapID
K01870	IARS, ileS	EC:6.1.1.5	Aminoacyl-tRNA biosynthesis	map00970

Gene Ontology

biological_process

GO:0006418 tRNA aminoacylation for protein translation

GO:0006428 isoleucyl-tRNA aminoacylation

cellular_component
GO:0005737 cytoplasm

molecular_function

GO:0000166 nucleotide binding

GO:0002161 aminoacyl-tRNA editing activity

GO:0003824 catalytic activity

GO:0004812 aminoacyl-tRNA ligase activity

GO:0004822 isoleucine-tRNA ligase activity

GO:0005524 ATP binding

Pfam domain

Protein	Pfam accession	Pfam name	Alignment start	Alignment end	E-value
Glyur000152s00012715.1	PF00133.17	tRNA-synt_1	113	168	7.80E-16
Glyur000152s00012715.1	PF00133.17	tRNA-synt_1	171	730	4.40E-153
Glyur000152s00012715.1	PF08264.8	Anticodon_1	776	940	5.40E-29
Glyur000152s00012715.1	PF06827.9	zf-FPG_IleRS	1010	1039	1.60E-07

Expression pattern

SRP053019

SRA Experiment	Description	TPM
SRR1783599	control	3.246
SRR1783600	control	3.262
SRR1783602	moderate drought stress	3.709
SRR1811619	moderate drought stress	3.589

DRP000996

SRA Experiment	Description	TPM
DRR006519	Root Summer glycyrrhizin high producing strain	8.250
DRR006520	Root Summer glycyrrhizin high producing strain	7.908
DRR006521	Root Winter glycyrrhizin high producing strain	6.186
DRR006522	Root Winter glycyrrhizin high producing strain	6.108
DRR006523	Root Summer glycyrrhizin low producing strain	6.604
DRR006524	Root Summer glycyrrhizin low producing strain	6.620
DRR006525	Leaf Summer glycyrrhizin high producing strain	22.011
DRR006526	Leaf Summer glycyrrhizin high producing strain	21.756

SRP065514

SRA Experiment	Description	TPM
SRR2867873	Salt Stress (root)	10.891
SRR2868004	Drought Stress (root)	9.994
SRR2967015	Control (root)	9.222

SRP215420

SRA Experiment	Description	TPM
SRR9715738	Leaf	16.086
SRR9715740	Leaf	26.362
SRR9715741	Leaf	14.411
SRR9715739	Root	1.126
SRR9715742	Root	3.555
SRR9715743	Root	2.652

SRP188776

SRA Experiment	Description	TPM
SRR8749027	MeJA induced 9h	7.111
SRR8749028	MeJA induced 9h	9.642
SRR8749029	MeJA induced 9h	4.510
SRR8749030	Uninduced	2.921
SRR8749031	Uninduced	1.280
SRR8749032	Uninduced	1.351

SRP175571

SRA Experiment	Description	TPM
SRR8400026	NaCl treatment - Root	11.462
SRR8400027	Control - Root	10.114